The technology makes it possible to simulate the ageing process of cells and at the same time trigger their rejuvenation. CRISPR screens are used to identify genes that are significantly responsible for ageing and rejuvenation. This innovative method has the potential to develop new therapeutic approaches to combat age-related diseases and to slow down ageing itself.

Building on this vision, Clock.bio focuses on not only understanding the biological mechanisms of ageing, but also reversing them. At the center of the research are human induced pluripotent stem cells (hiPSCs), which are not only able to remain youthful, but also to rejuvenate themselves after artificially induced aging. This unique ability of stem cells to perform a "reset" is being used by Clock.bio to develop new, effective approaches for the treatment of age-related diseases.

While all somatic cells age irreversibly, stem cells have the ability to turn back the clock. Clock.bio works with human induced pluripotent stem cells (iPSCs) and has developed a proprietary aging model that forces stem cells to age, mimicking the known cellular hallmarks of aging. This intervention triggers a self-rejuvenation mechanism in which iPSCs repair these hallmarks and become young and healthy again. Through a genome-wide CRISPR screen and single-cell RNA sequencing of over 3 million cells, generating 20 terabytes of data, clock.bio was able to decipher the "rejuvenation mechanism" and identify more than 100 genes that together form an "atlas of rejuvenation factors".